Generator

Part:BBa_K2796055:Design

Designed by: Tuoyu Zhou   Group: iGEM18_LZU-CHINA   (2018-10-07)


pGAL1-(hsa-miR-769-5p)-WPRE-SV40 poly(A) signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 512
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1088
    Illegal AgeI site found at 70
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 481


Design Notes

We need to consider how to use lentiviral vectors to enhance gene expression in cells. So we introduced the WPRE component.


Source

NCBI

References